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Once your drone frames are full and capped don’t automatically freeze or cut the brood out. Instead, use a cappings scratcher to remove and count the number of mites in several different areas on the comb. Early in the year it’s not uncommon to find only a few mites, occasionally you won’t find any. Possibly the rearing colony simply has a low mite count coming out of winter, or perhaps there’s a resistance factor involved. Either way, return the frame to the colony and allow that round of drones to emerge. Drones from low mite count colonies are a good thing. Use them at every opportunity. If you are really serious about controlling Varroa without the use of chemicals, genetic selection is the only route available. You have to start that selection process somewhere. Preserving or propagating drones from low mite count colonies is as good a place as any.